BBa_K1131001

BBa_K1131001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1131001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ramya Prathuri

Date created: 2013-09-09 11:00:00

Date modified: 2015-05-08 01:09:20

Mutant Flavin-containing monooxygenase (FMO)
M. aminisulfidivorans

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1131001_sequence (Version 1)

Description

As a corollary to the FMO from M. aminisulfidivorans submitted, this part has three specific mutations at F179A, G181A, F189A that eradicate FMO activity. This specific FMO converted indole to indoxyl which then oxidized to indigo, the well known dye. With these mutations, indigo was not observed, and was used as a control in several studies. This part contains the ORF of the mutated FMO only.

Notes

none

Source

Source is M. aminisulfidivorans genome, the mutations were cited by Kim et al, 2003.

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1131001/1

Member of these Collections

• iGEM Parts Registry