Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Toulouse
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:20
PhiC31 integrase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1132009_sequence (Version 1) |
Description
Isolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) encodes a serine-type recombinase that mediates the sequence-specific recombination between two different attachment sites, called attB and attP, which share a 3 bp central region, where the crossover occurs (Thorpe et al., 2000). Because the two sites recognized by the PhiC31 integrase differ and the recombination event leads to two different sites (attR and attL), PhiC31 based switch is unidirectional and definitive, except if the required excisionase factor is present. Recombination occurs irrespective of whether the substrate is supercoiled or linear, and does not require anything more than the integrase and attB, attP sites . (HELENA M. THORPE AND MARGARET C. M. SMITH, In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvaseyinvertase family, 1998).The recombination sites can be designed differently (position ??? orientation) in order to obtain a DNA 180?? inversion or an integration of the desired DNA sequence. The 180?? switch permits to design a lot of regulation tools, such as logical gates that can be found here. (BBa_K1132003, BBa_K1132004)
attB + attP + integrase → attR + attL + integrase
http://2013.igem.org/wiki/images/1/11/PhiC31.png
Notes
Isolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) encodes a serine-type recombinase that mediates the sequence-specific recombination between two different attachment sites, called attB and attP, which share a 3 bp central region, where the crossover occurs (Thorpe et al., 2000). Because the two sites recognized by the PhiC31 integrase differ and the recombination event leads to two different sites (attR and attL), PhiC31 based switch is unidirectional and definitive, except if the required excisionase factor is present. Recombination occurs irrespective of whether the substrate is supercoiled or linear, and does not require anything more than the integrase and attB, attP sites . (HELENA M. THORPE AND MARGARET C. M. SMITH, In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvaseyinvertase family, 1998).The recombination sites can be designed differently (position ??? orientation) in order to obtain a DNA 180?? inversion or an integration of the desired DNA sequence. The 180?? switch permits to design a lot of regulation tools, such as logical gates that can be found here. (BBa_K1132003, BBa_K1132004)
attB + attP + integrase → attR + attL + integrase
http://2013.igem.org/wiki/images/1/11/PhiC31.png