Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K1132010_sequence (Version 1)
|
Description
The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination.
The 180?? switch permits to design a lot of regulation tools, such as logical gates that can be found here (http://parts.igem.org/Part:BBa_K1132001, http://parts.igem.org/Part:BBa_K1132002).
attB + attP + integrase → attR + attL + integrase
http://2013.igem.org/wiki/images/3/37/TP901.png
Notes
The site-specific recombination system of temperate lactococcal bacteriophage TP901-1 integrase mediates site-specific recombination system. Originally from temperate lactoccocal bacteriophage TP901-1, this is a serine-type integrase able to invert, integrate or excise a DNA fragment according to the position and orientation of its specific recognition sites, attB and attP. This process is directional and definitive because of the transformation of attB and attP into attL and attR during recombination.
The 180?? switch permits to design a lot of regulation tools, such as logical gates that can be found here (http://parts.igem.org/Part:BBa_K1132001, http://parts.igem.org/Part:BBa_K1132002).
attB + attP + integrase → attR + attL + integrase
http://2013.igem.org/wiki/images/3/37/TP901.png
Source
later