Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Toulouse
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:21
Cph8 (Cph1/EnvZ fusion) under TetR repressible promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1132015_sequence (Version 1) |
Description
Chimeric Cph1 light receptor/EnvZ protein requires phycocyanobilin (PCB) biosynthetic genes for PCB formation (ho1 and PcyA). The exposure to red light (660nm) inhibits the activity of the EnvZ histidine kinase domain. In the dark, the EnvZ histidine kinase phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter. This part must be used in E.coli deficient in wild-type EnvZ.The promoter p(TetR) is constitutively on and repressed by TetR (Part:BBa_P0440). TetR binds to the p(TetR) and inhibits its operation. It regulates the light sensor Cph8. Tetracycline or aTc (anhydrotetracycline) binds to TetR and allows Cph8 production.
This part can be used with Part:BBa_K1132013 composed of the heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and tetracycline repressor (TetR) under constitutive promoter. Both of these parts form a red light responsive system, under tetR regulation.
Notes
Chimeric Cph1 light receptor/EnvZ protein requires phycocyanobilin (PCB) biosynthetic genes for PCB formation (ho1 and PcyA). The exposure to red light (660nm) inhibits the activity of the EnvZ histidine kinase domain. In the dark, the EnvZ histidine kinase phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpC promoter. This part must be used in E.coli deficient in wild-type EnvZ.The promoter p(TetR) is constitutively on and repressed by TetR (Part:BBa_P0440). TetR binds to the p(TetR) and inhibits its operation. It regulates the light sensor Cph8. Tetracycline or aTc (anhydrotetracycline) binds to TetR and allows Cph8 production.
This part can be used with Part:BBa_K1132013 composed of the heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and tetracycline repressor (TetR) under constitutive promoter. Both of these parts form a red light responsive system, under tetR regulation.
Source
later| Access | Instance | Definition |
| public public | BBa_R0040 BBa_K592018 | p(tetR) BBa_K592018 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_K592018 | 1,54 63,2318 | p(tetR) BBa_K592018 |