BBa_K1137003

BBa_K1137003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1137003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Deyell
Date created: 2013-10-03 11:00:00
Date modified: 2015-05-08 01:09:21

pSB9S1



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K1137003_sequence (Version 1)

Description

Contains the mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with CloDF13 replicon with a copy number of 20 ??? 40 per cell. The cat gene has been replaced with aadA conveying spectinomycin and streptomycin resistance.

Notes

The terminators in the pSB1C3 made gibson assembly difficult and as such we switched to traditional cloning using XhoI and AvrII cut sites.

Source

Upgraded pSB1C3 swapping out the origin and resistance cassettes for CloDF13 and aadA from pCDF

Sequence Annotation Location Component / Role(s)
aadA
CloDF13 ori
terminator
terminator
terminator
mRFP
prefix
stem loop
terminator
suffix
122,910
1050,1788
1705,1726
1762,1784
1887,1927
2177,2854
1929,1949
2986,3005
3041,3110
3021,3040
feature/protein CDS
sequence_feature feature/dna
feature/stem_loop stem_loop
stem_loop feature/stem_loop
stem_loop feature/stem_loop
CDS feature/cds
engineered_region feature/BioBrick
feature/stem_loop stem_loop
feature/stem_loop stem_loop
feature/BioBrick engineered_region
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1137003/1