Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Deyell
Date created: 2013-10-03 11:00:00
Date modified: 2015-05-08 01:09:21
pSB3C7
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K1137004_sequence (Version 1) |
Description
pSB3C7 ??? contains mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with P15A replicon with a copy number of 10-12 per cell. The cat gene has been maintained conveying chloramphenicol resistance.Notes
Designed to be co-expressed with pSB9S1, pSB10K1, and/or pSB6A3Source
Sequence comes from pSB1C3 with the ori and resistance changed form that of pACYC.| Sequence Annotation | Location | Component / Role(s) |
| Prefix mRFP stem loop Suffix Terminator Terminator p15A oriR CAT Terminator Terminator | 1,19 248,925 1057,1076 1092,1111 1137,1205 1226,1266 1288,2112 2478,3137 3367,3438 3459,3536 | engineered_region feature/BioBrick CDS feature/cds stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/stem_loop stem_loop feature/misc sequence_feature feature/protein CDS feature/stem_loop stem_loop feature/stem_loop stem_loop |