| Types | DnaRegion
|
| Roles | Device
engineered_region
|
| Sequences | BBa_K1137009_sequence (Version 1)
|
Description
A small RNA that inhibits Kanamycin resistance in E. coli. Contains PR promotor, sRNA, Scaffold from MicC and T1/TE. sRNA can be modified quickly and easily via site specific mutagenesis with primers.
Notes
The Sequence of the sRNA must match exactly the target sequence for silencing. Therefore we needed to sequence the available Chloramphenicol cassette to design our sRNA. If users Kanamycin cassette has any point mutations, silent or otherwise, silencing may not occur.
Source
gBlock Synthesis with sRNA design from "Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli" Yoo et al 2013 Nature Protocols
