BBa_K1137009

BBa_K1137009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1137009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Deyell
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:21

sRNA anti Kan



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K1137009_sequence (Version 1)

Description

A small RNA that inhibits Kanamycin resistance in E. coli. Contains PR promotor, sRNA, Scaffold from MicC and T1/TE. sRNA can be modified quickly and easily via site specific mutagenesis with primers.

Notes

The Sequence of the sRNA must match exactly the target sequence for silencing. Therefore we needed to sequence the available Chloramphenicol cassette to design our sRNA. If users Kanamycin cassette has any point mutations, silent or otherwise, silencing may not occur.

Source

gBlock Synthesis with sRNA design from "Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli" Yoo et al 2013 Nature Protocols

igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1137009/1