Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicolas Koutsoubelis, Anne L??chner
Date created: 2013-10-03 11:00:00
Date modified: 2015-05-08 01:09:21
tracRNA-CAS9
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1137014_sequence (Version 1) |
Description
This complex part contains the tracrRNA and the CAS9 under the control of constitutive promoters. Note that there is no RBS before the tracRNA. The tracrRNA-CAS9 part is the heart of the CRISPR system. The tracrRNA and the corresponding target crRNA dimerize and are processed and hybridize before binding to the CAS9 protein. The tracrRNA has no specific part that is responsible for binding to the targeted DNA. Therefor this part can be used together with any crRNA. The CAS9 gene encodes for the CRISPR associated protein 9 that is needed to generate a double strand break at a target site. CAS9 binds to the tracRNA-crRNA dimer/gRNA and is then guided by them/it to the target site where it generate a double strand break. This part can be used together with the crRNA anti KAN or the gRNA anti KAN and cause double strand breaks. It can of course also be used together with new generated crRNAs/gRNAs. The tracRNA-CAS9 part is taken from the CRISPER plasmid of the Jiang et al. 2013 paper.Notes
-Source
The plasmid was ordered from Addgene and come originally from the paper below:Wenyan Jiang, David Bikard, David Cox, Feng Zhang & Luciano A Marraffini (2013): RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nature Biotechnology 31 (3), 233-239.