| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1137015_sequence (Version 1)
|
Description
This part is a reporter element of our sensor system that senses double strand breaks and consists of the native pRecA promoter and RBS sequence like in EcoCyc. The promoter and the RBS are fused to the full LacZ gene (I732017) that encodes for ??-galactosidase. The RecA protein is a recombinase that is active during the SOS response after double strand breaks. RecA and its promoter are well characterized and used in many SOS studies. ??-galcatosidase cleaves lactose and its derivate X-Gal. X-Gal is cleaved into galactose and an insoluble blue dye. Therefore the LacZ gene is a classical reporter gene.
Notes
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Source
The promoter was synthesized by IDT. The LacZ gene was amplified from the Addgene plasmid pBAC-BA-lacZ.
