Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Naoki Watarai
Date created: 2013-08-31 11:00:00
Date modified: 2015-05-08 01:09:21
Promoterless-M13 on pSB3
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1139019_sequence (Version 1) |
Description
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.This biobrick is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is deleted.The capsid is formed only when g2p(gene 2 protein) is expressed.
You can insert the target gene or circuit on the downstream of the Lac promoter.
Notes
This biobrick is derived from M13mp18 phage vector, amplified by using PCR.Source
TBD| Sequence Annotation | Location | Component / Role(s) |
| g2p rbs g2p g10p g5p g7p g9p g8p g3p g6p g1p g11p g4p packaging sequence M13 origin | 1,6 13,1247 1135,1247 1257,1520 1522,1620 1620,1715 1715,1936 1993,3364 3270,3605 3610,4653 4330,4653 4634,5911 5913,5963 6047,6225 | feature/rbs ribosome_entry_site CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein CDS feature/protein feature/protein CDS CDS feature/protein feature/stem_loop stem_loop feature/stem_loop stem_loop |