BBa_K1140002

BBa_K1140002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1140002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Janssel Reyes del Castillo
Date created: 2013-09-07 11:00:00
Date modified: 2015-05-08 01:09:23

pLac + 32 oC RNA thermometer + GFP(LVA)



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1140002_sequence (Version 1)

Description

A Green fluorescent protein (with LVA tag) that is regulated by a 32 degrees riboswitch. The part includes a the promoter "pLac".

Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. Promoter pLac.
Based on a fusion of a RBS and a Riboswitch. GFP (LVA). T7 double terminator(Further information coming soon).

Sequence Annotation Location Component / Role(s)
pLac
RNA thermometer
GFP+LVA
T7 terminator
1,56
65,147
154,912
920,964
promoter feature/promoter
feature/stem_loop stem_loop
CDS feature/cds
stem_loop feature/stem_loop
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1140002/1