Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Silvia J Ca??as
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:26
GAL4 (3UAS) activation reporter
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1144006_sequence (Version 1) |
Description
The level of expression from the GAL1 promoter (Saccharomyces cerevisiae) is enhanced due to the presence of UAS sites. We fused a modified pGAL1 (3UAS) with a KOZAC sequence, mCherry and the HHO1 yeast terminator to asses the level of expression due to the activacion of GAL4 binding domainNotes
NoneSource
pGAL1(3UAS) was synthetized(BBa_K1144002) and fused with the KOZAC sequence using Phusion PCR. Parts BBa_J06504 and BBa_K530019 were obtained from the 2013 Distribution kit and fused using Phusion PCR| Access | Instance | Definition |
| public public public | BBa_K1144002 BBa_J06504 BBa_K530019 | BBa_K1144002 mCherry BBa_K530019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1144002 BBa_J06504 BBa_K530019 | 1,482 489,1202 1211,1522 | BBa_K1144002 mCherry BBa_K530019 |