BBa_K1144006

BBa_K1144006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1144006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Silvia J Ca??as
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:26

GAL4 (3UAS) activation reporter



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1144006_sequence (Version 1)

Description

The level of expression from the GAL1 promoter (Saccharomyces cerevisiae) is enhanced due to the presence of UAS sites. We fused a modified pGAL1 (3UAS) with a KOZAC sequence, mCherry and the HHO1 yeast terminator to asses the level of expression due to the activacion of GAL4 binding domain

Notes

None

Source

pGAL1(3UAS) was synthetized(BBa_K1144002) and fused with the KOZAC sequence using Phusion PCR. Parts BBa_J06504 and BBa_K530019 were obtained from the 2013 Distribution kit and fused using Phusion PCR

igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1144006/1