Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margarita Kopniczky
Date created: 2013-09-07 11:00:00
Date modified: 2015-05-08 01:09:26
Promoter J23104 - RBS B0034 - amilCP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1149020_sequence (Version 1) |
Description
The chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe.The blue coloration made it very easy to select the ligations with the insert from the transformation plate:
http://igem.org/wiki/images/0/01/AmilCPligICL1.JPG
http://igem.org/wiki/images/f/f4/AmilCPligICL2.JPG
We were inspired by the beautiful blue coral colour and taught the bacteria how to write our name with it.
http://igem.org/wiki/images/f/fb/PlasticityBluePlate.JPG
We have observed change in colour of an Overnight culture with the construct. However, it was not as blue as the previous results from Uppsala 2011. http://parts.igem.org/wiki/images/a/a8/On_cultures_BYR_small.jpg
http://igem.org/wiki/images/f/f5/AmilCP_cultureICL.jpg
We have verified the sequence and detected 3 mutations compared to the expected results. However, these are located after the prefix and at the scar site and do not affect the coding region or the function of the construct.
Notes
This is a composite part from BBa_K608002 (http://parts.igem.org/Part:BBa_K592009) and BBa_K592009 (http://parts.igem.org/Part:BBa_K608002). It is functionally similar to BBa_K592031 (http://parts.igem.org/Part:BBa_K592031) which has a J23101 promoter instead of the J23104 but contains the same RBS and amilCP.Source
Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.| Access | Instance | Definition |
| public public | BBa_K608002 BBa_K592009 | BBa_K608002 amilCP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K608002 BBa_K592009 | 1,55 62,730 | BBa_K608002 amilCP |