| Types | DnaRegion
|
| Roles | RNA
mature_transcript_region
|
| Sequences | BBa_K115005_sequence (Version 1)
|
Description
This part works as a thermoswitch the same as BBa_K115001. At low temperatures the RNA is folded into a structure that inhibits the Shine Dalgarno sequence. When the temperature rises, the RNA unfolds and the Shine Dalgarno sequence becomes exposed so that translation of the protein following this RNA can initiate.
Notes
In contrast to BBa_K115001 a His-tag is added at the 3' end of the thermoswitch. We did this to shift the scar forward, otherwise the scar would alter the original RNA sequence. In case of part BBa_K115001 we left the scar inside the thermoswitch and altered the sequence in such a way that the predicted secundary structure remained the same.
Source
This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein.
