BBa_K115005

BBa_K115005 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K115005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bastiaan van den Berg
Date created: 2008-07-28 11:00:00
Date modified: 2015-05-08 01:09:28

Temperature sensitive 5



Types
DnaRegion

Roles
RNA

mature_transcript_region

Sequences BBa_K115005_sequence (Version 1)

Description

This part works as a thermoswitch the same as BBa_K115001. At low temperatures the RNA is folded into a structure that inhibits the Shine Dalgarno sequence. When the temperature rises, the RNA unfolds and the Shine Dalgarno sequence becomes exposed so that translation of the protein following this RNA can initiate.

Notes

In contrast to BBa_K115001 a His-tag is added at the 3' end of the thermoswitch. We did this to shift the scar forward, otherwise the scar would alter the original RNA sequence. In case of part BBa_K115001 we left the scar inside the thermoswitch and altered the sequence in such a way that the predicted secundary structure remained the same.

Source

This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein.

Sequence Annotation Location Component / Role(s)
start
SD
His-tag
103,105
90,95
109,138
start_codon feature/start
ribosome_entry_site feature/rbs
tag feature/tag
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K115005/1