BBa_K115006

BBa_K115006 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K115006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bastiaan van den Berg
Date created: 2008-07-28 11:00:00
Date modified: 2015-05-08 01:09:28

Temperature sensitive 5



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K115006_sequence (Version 1)

Description

This part works as a thermoswitch the same as BBa_K115002. At low temperatures the RNA is folded into a structure that inhibits the Shine Dalgarno sequence. When the temperature rises, the RNA unfolds and the Shine Dalgarno sequence becomes exposed so that translation of the protein following this RNA can initiate.

Notes

In contrast to BBa_K115002 a His-tag is added at the 3' end of the thermoswitch. We did this to shift the scar forward, otherwise the scar would alter the original RNA sequence. In case of part BBa_K115002 we left the scar inside the thermoswitch and altered the sequence in such a way that the predicted secundary structure remained the same.

Source

Salmonella Enterica Tyhpy (CP000886.1)

Sequence Annotation Location Component / Role(s)
start
SD
His-tag
59,61
47,52
65,94
feature/start start_codon
ribosome_entry_site feature/rbs
tag feature/tag
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K115006/1