Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis
Date created: 2013-09-18 11:00:00
Date modified: 2015-05-08 01:09:29
IndC Indigoidine Synthetase (cds)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1152008_sequence (Version 1) |
Description
The IndC Indigoidine Synthetase from Photorhabdus luminescens laumondii TT01 is a nonribosomal peptide synthetase (NRPS) which produces the blue pigment indigoidine. Indigoidine itself consists of two cyclic glutamines. IndC is a single module NRPS and consists of an Adenylation-, Thiolation- and a Thioesterase-domain. The A-domain thus contains an internal Oxidation domain.Notes
The native indC contains an internal EcoRI and SpeI cutting site which was mutated using a CPEC approach.Source
The coding sequence was amplified from Photorhabdus luminescens laumondii TT01 DSM15139.| Sequence Annotation | Location | Component / Role(s) |
| indC indigoidine synthetase (cds) silent mutation a-to-g silent mutation t-to-g | 1,3855 212,212 513,513 | feature/cds CDS feature/s_mutation point_mutation point_mutation feature/s_mutation |