Types | DnaRegion
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Roles | engineered_region
Composite
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Description
pccB-accA2 pathway from Streptomyces coelicolor producing methylmalonyl-CoA, the sfp 4'-pptase, and a permeability device.
Notes
The permeability device is toxic to the cells, therefore it is driven by a weak promoter and a weak RBS.
Source
pccB-accA2: thanks to Mathew Mattozzi for providing his construct. Sfp: amplified from E. coli BAP1, genome, originally from Bacillus subtilis. Permeability device: 2012 parts distribution.