P(narG)

BBa_K1155005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1155005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Solenne Ithurbide, Abdou, Ana??s, Zhu, Xavier

Date created: 2013-09-12 11:00:00

Date modified: 2015-05-08 01:09:30

Promoter of narG activated by FNR in anaerobic conditions

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1155005_sequence (Version 1)

Description

Promoter of gene narG from E. coli. Activated by FNR in anaerobic conditions and inactivated in aerobic conditions.

The promoter of the gene nirB is regulated by FNR. It's a class II promoter with two FNR boxes. (Scott et al, FEBS letter, 2003)

Aerobic conditions: +O2 --> FNR inactivated --> Promoter non activated / Anaerobic conditions: - O2 --> FNR activated --> Promoter activated

Notes

We conserved all the promoter region until the last nucleotide before narG ORF and approximately 100 bp before FNR boxes

Source

Escherichia coli K-12 substr. MG1655, web site EcoCyc

http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10653

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
FNR box I	34,38	operator, feature/operator
FNR box II	43,48	operator, feature/operator

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1155005/1

Member of these Collections

• iGEM Parts Registry