Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-13 11:00:00
Date modified: 2015-05-08 01:09:31
Autolysis resistant TEV Protease S219V mutant in RFC[25]
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1159100_sequence (Version 1) |
Description
TEV Protease from Tabacco Etch Virus (TEV) is a highly specific protease that cleaves the sequence "Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S))" very specifically before the last Gly/Ser. The wildtype version of the protease has a short half-life due to self-inactivation through autolysis. This mutant S219V is autolysis resistant and its enzymatic activity is not affected by the mutation.Notes
xSource
Tabacco Etch Virus| Sequence Annotation | Location | Component / Role(s) |
| Ser --> Val for autolysis resistance | 655,657 | sequence_alteration feature/mutation |