BBa_K1159100

BBa_K1159100 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1159100
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-13 11:00:00
Date modified: 2015-05-08 01:09:31

Autolysis resistant TEV Protease S219V mutant in RFC[25]



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1159100_sequence (Version 1)

    Description

    TEV Protease from Tabacco Etch Virus (TEV) is a highly specific protease that cleaves the sequence "Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S))" very specifically before the last Gly/Ser. The wildtype version of the protease has a short half-life due to self-inactivation through autolysis. This mutant S219V is autolysis resistant and its enzymatic activity is not affected by the mutation.

    Notes

    x

    Source

    Tabacco Etch Virus

    Sequence Annotation Location Component / Role(s)
    Ser --> Val for autolysis resistance
    		655,657
    		sequence_alteration feature/mutation

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1159100/1
     

    Attachments

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