Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:31
N-TEV-Protease_36AALinker_PIF3 (Second part for a light-switchable TEV-Protease) in RFC[25]
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1159107_sequence (Version 1) |
Description
This parts encodes a fusion protein consisting the N-terminal half of the TEV Protease as its terminus and first 100 amino acids of PIF3 as its C-terminus. PIF3 interacts with PhyB in the presence of red light. This reaction is reversible by far-red light. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions.By expressing this part together with the second essential protein BBa_K1159106 that contains the part, which interacts with PIF3, and the C-terminal half of the splitted TEV Protease, they form together a light-switchable TEV Protease system which can be activated by red light and inactivated by far-red light.
Notes
xSource
Synthetic| Access | Instance | Definition |
| public public public public public | BBa_K1159101 BBa_K1159993 BBa_K243029 BBa_K1159993 BBa_K1159103 | BBa_K1159101 BBa_K1159993 GSATLinker BBa_K1159993 BBa_K1159103 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1159101 BBa_K1159993 BBa_K243029 BBa_K1159993 BBa_K1159103 | 1,354 355,360 361,468 469,474 475,771 | BBa_K1159101 BBa_K1159993 GSATLinker BBa_K1159993 BBa_K1159103 |