BBa_K1159115

BBa_K1159115 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1159115
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:31

Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based) with downstream IRES



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Translational_Unit

    Sequences BBa_K1159115_sequence (Version 1)

    Description

    This part encodes a light-switchable TEV Protease system consisting of two fusion proteins (BBa_K1159106 and BBa_K1159107) seperated by an polioviral IRES BBa_K1159300 which allows polycistronic expression in eukaryotic systems. You can easily express a third protein by simply clone your desired protein in the suffix of this part because this part ends with a polioviral IRES which allows cap-independent translation. After addition of your desired protein you can express this system by inserting your favored promoter/terminator in the prefix/suffix your construct. For further information, please see under the subparts linked in the text.

    Notes

    x

    Source

    Synthetic

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1159115/1
     

    Attachments

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