BBa_K1159200

BBa_K1159200 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1159200
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-13 11:00:00
Date modified: 2015-05-08 01:09:31

Splitted and engineered N-terminal FbaB for isopeptide bound formation (SpyCatcher) in RFC[25]



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1159200_sequence (Version 1)

Description

This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide. That oligopeptide can be found under BBa_K1159201. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Notes

x

Source

Streptococcus pyogenes

Sequence Annotation Location Component / Role(s)
N-terminal FbaB (modified SpyCatcher)
Iso --> Glu
Met --> Tyr
Reactive Lys for isopeptide forming reaction
1,339
100,102
205,207
91,93
feature/protein CDS
sequence_alteration feature/mutation
feature/mutation sequence_alteration
feature/conserved conserved_region
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1159200/1