Vio B

BBa_K1161002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1161002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Esau
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:09:32

Violacein B



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1161002_sequence (Version 1)

Description

Violacein B (Vio)B is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.

Notes

See above

Source

See above

Sequence Annotation Location Component / Role(s)
B0030
Spe/Pst
cds
1,15
16,21
22,3028
feature/rbs ribosome_entry_site
feature/misc sequence_feature
feature/cds CDS
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BBa_K1161002/1