BBa_K1162013

BBa_K1162013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1162013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Miller
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:32

promoter+rbs+N terminal His Tag and GFP



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1162013_sequence (Version 1)

    Description

    This part demonstrates that the N-terminal 10x His Tag functions as desired. It contains a promoter and rbs (BBa_K208010) with a GFP fusion (BBa_K208000). His Tag and GFP are RFC 23 compatible which allows for protein fusion with the His Tag.

    Notes

    RFC 23 when making the N terminal His Tag and GFP fusion.

    Source

    E. coli.

    Access Instance Definition
    public
    public
    		BBa_K1162010
    BBa_K208000
    		BBa_K1162010
    BBa_K208000

    Sequence Annotation Location Component / Role(s)
    BBa_K1162010
    BBa_K208000
    		1,259
    266,985
    		BBa_K1162010
    BBa_K208000

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1162013/1
     

    Attachments

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