Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guillaume Mercy
Date created: 2013-09-01 11:00:00
Date modified: 2015-05-08 01:09:32
Inverter composed of Fur Binding site from AceB promoter + LacI-LVA
| Types | DnaRegion |
| Roles | Inverter engineered_region |
| Sequences | BBa_K1163999_sequence (Version 1) |
Description
FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron FUR will be activated and it modification of conformation will induce a dimerization of two FUR. When the FUR protein is dimerized, it will bindind to the DNA in a Fur Binding Site, then FUR will inhibit the mRNA transcription.To realize our project we want to use this Fur binding site merged with an inhibitor (here LacI) to create an inverter system.
AceB regulatory promoter will decrease the expression of the LacI-LVA gene (LacI protein with a LVA degredation tag) when the FUR protein is dimerized in the presence of iron.
This part should be used to express a gene of interest that is under control of a Lac operator (LacO). This way, the gene will be positively regulated in the presence of iron.
Notes
...Source
AceB promoter sequence has been extracted by PCR from E.coli genome.| Access | Instance | Definition |
| public | BBa_K188141 | BBa_K188141 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K188141 | 1,1234 | BBa_K188141 |