BBa_K1164004

BBa_K1164004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1164004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wilson Lam, Dylan Siriwardena, Yara Abou-Hamde
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:32

pTRELX: Modified Gal1 promoter
gal4O replaced w/ tetO
repressable via lacO



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1164004_sequence (Version 1)

Description

This is a modified version of the native Gal1 promoter found in S.cerevisiae. The four gal4 binding domains present in the native promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites.

Notes

N/A

Source

S.cerevisiae/commercial synthesis

Sequence Annotation Location Component / Role(s)
pTRELX
tetO
tetO
tetO
tetO
TATA box
lacO
1,558
7,25
28,46
47,65
113,131
322,328
352,368
promoter feature/promoter
operator feature/operator
operator feature/operator
feature/operator operator
feature/operator operator
feature/misc sequence_feature
operator feature/operator
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1164004/1