Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wilson Lam, Dylan Siriwardena, Lloyd Mai, Yara Abou-Hamde
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:32
pTRE driving yEGFP with tPGK1 terminator
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1164007_sequence (Version 1) |
Description
This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites. Upon activation, yEGFP will be produced.Notes
N/ASource
S.cerevisiae/commercial synthesis| Sequence Annotation | Location | Component / Role(s) |
| pTRE tetO tetO tetO tetO TATA box yEGFP tPGK1 | 1,466 7,25 28,46 47,65 113,131 322,328 467,1180 1181,1374 | feature/promoter promoter feature/operator operator operator feature/operator operator feature/operator operator feature/operator sequence_feature feature/misc feature/cds CDS feature/misc sequence_feature |