BBa_K116402

BBa_K116402 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K116402
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chih-Hsien Yang
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:09:33

polyphosphate kinase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K116402_sequence (Version 1)

Description

gene ppk in E.coli, an synthase of Polyphosphate to store phosphate into Polyphosphate form

Notes

WT ppk gene in E.coli has a internal biobrick standard cutting site "GAA TTC" (EcoRI site). Thus we design an set of internal primers to replace the EcoRI site "GAA TTC" as "GAG TTT." This ppk mutant can be applied in biobrick standard assembly.

Source

E. coli K12 genomic DNA

Sequence Annotation Location Component / Role(s)
start
mutated EcoRI site
stop
1,3
268,273
2065,2067
feature/start start_codon
feature/misc sequence_feature
feature/stop stop_codon
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
rdf-schema#seeAlso
P0A7B2
 
rdf-schema#seeAlso
P0A7B1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K116402/1