HIP

BBa_K1166000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1166000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luis Mario Leal Garza
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:33

FNR-HIP1



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1166000_sequence (Version 1)

Description

Composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3??? downstream in a hypoxic environment.

Notes

The FNR gene has additional nucleotides in the 5' direction including the 3' end of the ORF upstream in the genomic sequence.

Source

FNR is a native E.coli gene that we had synthesized for our project. HIP1 promoter was also synthesized and belongs to Salmonella.

Sequence Annotation Location Component / Role(s)
HIP1
FNR Native Promoter
BBa_B0034
BBa_B0015
FNR
1168,1221
1,283
1222,1234
1049,1167
284,1033
promoter feature/promoter
feature/promoter promoter
feature/rbs ribosome_entry_site
engineered_region feature/BioBrick
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1166000/1