Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nguyen Xuan Hung
Date created: 2008-10-06 11:00:00
Date modified: 2015-05-08 01:09:34
ColicinE7 with Immunity
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K117001_sequence (Version 1) |
Description
This gene sequence once expressed will produce colicin E7. This is an endonuclease with random cutting site and can kill various strains of EHEC bacteria. However, inside the host cell, immediatle after being produced, its toxicity is inhibited by Immunity protein (which is also expressed at the same time). These 2 proteins form a complex which is harmless to the host cell.Under SOS response, the host cell produces lysis protein, which cause the cell to lyze. At the same time, lysis protein remove Immunity protein from the complex with colE7. As a result, colE7 regains its toxicity and ability to kill the EHEC bacteria nearby.
For information on lysis gene, please refer to our BBa_K117000 part.
Notes
to be updatedSource
to be updated| Sequence Annotation | Location | Component / Role(s) |
| caeE7 RBS caeE7 toxicity domain Immunity (ceiE7) | 117,124 478,587 1862,1899 | feature/rbs ribosome_entry_site feature/misc sequence_feature feature/cds CDS |