BBa_K1175007

BBa_K1175007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1175007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas K. Goldner
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:35

beta-galacturonidase (YesZ) from Bacillus Subtilis 168



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1175007_sequence (Version 1)

Description

yesZ
The enzyme acts on the terminal end of side chains of Rhamnogalacturonan I Pectin, releasing the free galactose. This gene has been isolated from the bacterium Bacillus subtilis subtilis 168.
The enzyme yesZ has beta-galactosidase (beta-galacturonidase) activity, cleaving (1→4)-β-D-galactans to produce single galactose molecules from RG I Pectin.

Usage and Biology
Pectin is a component of the primary cell wall of plants. As galactose can be metabolized by the human body and by animals, it is an alternative to the usual target of nutrition/energy boosts in biotechnology, glucose.

(1) http://www.ncbi.nlm.nih.gov/pubmed/17449691
(2) http://subtiwiki.uni-goettingen.de/wiki/index.php/YesZ
(3) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1694227/
(4) http://aem.asm.org/content/73/12/3803
(5) http://www.tandfonline.com/doi/full/10.1080/15583724.2011.615962#tabModule

Notes

No design considerations were needed, although we did add a second stop codon.

Source

Bacillus Subtilis 168 genomic DNA

Sequence Annotation Location Component / Role(s)
Start Codon
YesZ Enzyme
Double Stop Codon
1,3
4,1988
1989,1995
feature/start start_codon
CDS feature/protein
feature/stop stop_codon
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BBa_K1175007/1