Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K1175007_sequence (Version 1)
|
Description
yesZ
The enzyme acts on the terminal end of side chains of Rhamnogalacturonan I Pectin, releasing the free galactose. This gene has been isolated from the bacterium Bacillus subtilis subtilis 168.
The enzyme yesZ has beta-galactosidase (beta-galacturonidase) activity, cleaving (1→4)-β-D-galactans to produce single galactose molecules from RG I Pectin.
Usage and Biology
Pectin is a component of the primary cell wall of plants. As galactose can be metabolized by the human body and by animals, it is an alternative to the usual target of nutrition/energy boosts in biotechnology, glucose.
(1) http://www.ncbi.nlm.nih.gov/pubmed/17449691
(2) http://subtiwiki.uni-goettingen.de/wiki/index.php/YesZ
(3) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1694227/
(4) http://aem.asm.org/content/73/12/3803
(5) http://www.tandfonline.com/doi/full/10.1080/15583724.2011.615962#tabModule
Notes
No design considerations were needed, although we did add a second stop codon.
Source
Bacillus Subtilis 168 genomic DNA