BBa_K1178000

BBa_K1178000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1178000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Razan Alnahhas
Date created: 2013-08-25 11:00:00
Date modified: 2015-05-08 01:09:35

tRNA and synthetase for 3,4-dihydroxy-L-phenylalanine (L-DOPA) incorporation at UAG codon



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1178000_sequence (Version 1)

Description

The part includes promoters and a terminator along with the gene coding for the DOPA synthetase and the tRNA for DOPA. Together this part incorporates L-DOPA during translation at AUG Amber stop codons.

Notes

The synthetase gene is codon optimized for E. coli.

Source

The synthetase and tRNA were synthetically made.

Sequence Annotation Location Component / Role(s)
promoter
DOPA synthetase
promoter
tRNA
terminator
74,220
234,1154
1331,1387
1399,1473
1498,1527
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
igem#experience
Issues
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1178000/1