| Types | DnaRegion
|
| Roles | Signalling
engineered_region
|
| Sequences | BBa_K1180003_sequence (Version 1)
|
Description
Lamp 2b (lysosomal associated membrane protein 2b) is a protein ubiquitously expressed on the surface of the exosomes. By genetically engineer a target protein to the outmembrane part of the lamp 2b, we can use the lamp 2b to bring our target protein to the surface of the exosome. Thus we can endow the exosome with site-specificity by the addition of the fusion protein.For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study, we know that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells.
Notes
We tried to make the pre-S1 sequence as short as possible in order to avoid any immune response which might be induced by the fusion protein.
Source
The sequence of our part come from the protein lamp 2b and pre-S1 from HBV envelope protein.
