Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-07-23 11:00:00
Date modified: 2015-05-08 01:09:36
crtI coding sequence encoding phytoene dehydrogenase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K118003_sequence (Version 1) |
Description
This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.Notes
The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows:* crtImutf1: gtt tac agt aag gtt gcc
* crtImutf2: agt aac tct ctg ttt gtg c
In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
Source
Pantoea ananatis genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| PstI site removed PstI site removed crtI coding sequence | 478,483 889,894 1,1479 | feature/s_mutation point_mutation point_mutation feature/s_mutation CDS feature/cds |