BBa_K118011

BBa_K118011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K118011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-08-18 11:00:00
Date modified: 2015-05-08 01:09:36

PcstA (glucose-repressible promoter)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K118011_sequence (Version 1)

Description

This is the promoter for the Eschaerichia coli JM109 cstA gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (cstA encodes the carbon starvation protein.)

Notes

No special considerations

Source

Eschaerichia coli JM109 genomic DNA

Sequence Annotation Location Component / Role(s)
cAMP receptor protein binding site
RNA polymerase binding site
1,42
101,131
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K118011/1