Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-08-18 11:00:00
Date modified: 2015-05-08 01:09:37
rbs+crtE (geranylgeranyl pyrophosphate synthase)
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K118014_sequence (Version 1) |
Description
This is the coding sequence of crtE from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes geranylgeranyl pyrophosphate synthase, part of the carotenoid biosynthesis pathway, which converts farnesyl diphosphate to geranylgeranyl diphosphate.Notes
The crtE BioBrick (BBa_I742151) had to be transferred from the non-compliant BABEL vector to a compliant pSB1A2 vector.Source
The synthetic ribosome binding site was added by fusion PCR to the crtE gene, which was amplified from Pantoea ananatis DSMZ 30080 (ATCC 19321) genomic DNA.| Access | Instance | Definition |
| public public | BBa_K118012 BBa_I742151 | BBa_K118012 BBa_I742151 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K118012 BBa_I742151 | 1,4 11,922 | BBa_K118012 BBa_I742151 |