Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-10-05 11:00:00
Date modified: 2015-05-08 01:09:37
glgC16 (glgC with G336D substitution)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K118016_sequence (Version 1) |
Description
This is the coding sequence of glgC (ADP-glucose pyrophosphorylase) from Escherichia coli JM109 with the substitution G336D. This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung et al, 1986; Meyer et al., 1988)Notes
The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL.Source
Escherichia coli JM109 genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| G336D EcoRI site removed EcoRI site removed | 1006,1008 571,576 1068,1073 | sequence_alteration feature/mutation point_mutation feature/s_mutation feature/s_mutation point_mutation |