Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bill Heymann, Phil Jensen
Date created: 2013-08-13 11:00:00
Date modified: 2015-05-08 01:09:37
AmilCP screening part
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1188000_sequence (Version 1) |
Description
This part has a strong constitutive promoter (J23100) the standard RBS (B0034) and AmilCP (K592009). When assembled into a plasmid it gives a strong blue color.The reason we created this part is the default backones all have essentially the same construct but using RFP instead. With an insert with a different color it made it much easier to debug problems with digests, ligations and transformations. We also used it to try to find a problem with some strange background issues we had.
This was part also use to create a lot of AmilCP that was used in our protein purification experiments.
Notes
We needed a strong blue color to make it easy to distinguish this from a red carry through. We used a strong anderson promotor (J23100), standard RBS and hooked it to AmilCP.Source
J23100, K592009 and B0034 came from the registry.| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_B0034 BBa_K592009 | BBa_J23100 BBa_B0034 amilCP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K592009 | 1,35 44,55 62,730 | BBa_J23100 BBa_B0034 amilCP |