BBa_K119009

BBa_K119009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K119009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualp

Date created: 2008-09-28 11:00:00

Date modified: 2015-05-08 01:09:38

cI promoter RcnR op RcnA

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K119009_sequence (Version 1)

Description

This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion.

Notes

We needed to repress the RcnA activity, although niquel is present in the cell. In order to achieve this, we added cI promoter(BBa_R0051) (an operator) sequence which is regulated by cI.

Source

Constructed by LCG-UNAM-Mexico Team

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
OR2	5,21	non_covalent_binding_site, feature/binding
BBa_R0051	5,53	engineered_region, feature/BioBrick
-35	19,24	promoter, feature/promoter
OR1	29,45	feature/binding, non_covalent_binding_site
-10	42,47	feature/promoter, promoter
BBa_K119002	54,136	engineered_region, feature/BioBrick
start	137,139	start_codon, feature/start
RcnA	137,963	CDS, feature/cds
BBa_K119003	137,963	engineered_region, feature/BioBrick
TAA	959,961	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K119009/1

Member of these Collections

• iGEM Parts Registry