Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rohan Bendre, Aman Kumar, Namit Holay, Mitan Sutradhar
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:38
pLuxR --> I3A gene
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1194003_sequence (Version 1) |
Description
The I3A gene is actually a polycistronic construct composed of two genes coding for two enzymes - tryptophan transaminase and indole pyruvate decarboxylase. The two DNA constructs are joined by a linker sequence. This entire assembly is placed downstream of an N-acyl homoserine lactone (AHL) inducible promoter pLuxR.The I3A genes function to convert tryptophan to indole-3-acetaldehyde (I3A). Tryptophan transaminase converts tryptophan to indole pyruvate while indole pyruvate decarboxylase converts indole pyruvate to I3A. I3A is a known biofilm inhibitor.
In the future, other teams can use either of our two enzymes as there is a restriction enzyme site incorporated in the linker sequence. The part will be useful in any project involving biosynthesis of indole and its derivatives.
Notes
We wanted to make our part flexible by providing for easy separation of the polycistronic construct into the two component enzymes. For this purpose, we included the restriction enzyme site for Hinf1 in the linker sequence connecting the two genes.Source
Tryptophan transaminase is derived from Aarabidopsis thaliana.Indole pyruvate decarboxylase is derived from Azospirillum brasilense.