Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aman Kumar
Date created: 2013-09-25 11:00:00
Date modified: 2015-05-08 01:09:38
pLuxR --> ompF --> Gb3 mimic
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1194004_sequence (Version 1) |
Description
Gb3 mimic codes for a nine-amino acid peptide that has anti-Shiga toxin activity. It is coded downstream of ompF which is an periplasmic translocation signal peptide. The entire assembly is under control of an N-acyl homoserine lactone (AHL) dependent pLuxR promoter.The part, when translated, produces the Gb3 mimic peptide. The ompF signal peptide helps to transport Gb3 mimic into the extracellular space. Gb3 mimic neutralises the Shiga toxin by binding to it and sequestering it.
Other teams can utilise the ompF signal peptide if they want to ensure extracellular secretion of their protein of interest, especially if it is a small peptide. The Gb3 mimic peptide, though specific for the Shiga toxin, can be used for toxicology studies in general. Particularly, it can be of use to create a peptide library to investigate anti-toxin activities of therapeutic peptides by molecular docking.
Notes
The nine amino acid sequence had to be codon optimized for the optimal expression in E. coli.Source
OmpF is from Escherichia coli. Gb3 mimic is a synthetic peptide taken from peptide library. pLuxR is taken from the parts registry.| Sequence Annotation | Location | Component / Role(s) |
| Original Sequence for K1194004 | 1,235 | sequence_feature feature/misc |