BBa_K1197004

BBa_K1197004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1197004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alişan Kayab??len
Date created: 2013-09-19 11:00:00
Date modified: 2015-06-08 11:17:26

RBS SpoVG + LacI + DT



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1197004_sequence (Version 1)

    Description

    This is a LacI producing part which is proper for B.subtilis. Any promoter can be added to the downstream of that part to obtain a LacI coding device.

    Notes

    This part is necessary for our kill switch device. To produce LacI in B.subtilis, we designed primers which binds to LacI and have RBS SpoVG sequence as prefix together with RE cut sites.

    Source

    This part is obtained from BBa_I732820 part by changing the original RBS sequence with RBS SpoVG via specific primers.

    Access Instance Definition
    public
    public
    public
    		BBa_K606061
    BBa_I732100
    BBa_B0015
    		BBa_K606061
    LacI
    BBa_B0015

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1197004/1
     

    Attachments

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