BBa_K1202106

BBa_K1202106 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1202106
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: safa tapan
Date created: 2013-09-23 11:00:00
Date modified: 2015-05-08 01:09:40

TAT-HA2-Apoptin: Cancer killing peptide



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K1202106_sequence (Version 1)

Description

this part has three component
Apoptin: forcing cancer cell to apoptosis
Tat:signal peptide
Ha:signal peptide

Notes

Due to the rare codon analysis, codon optimization is performed for this part. Codons are optimized for E.coli.

Source

tat signal peptide was prepared from human immunodeficiency virus-1(HIV-1)

Sequence Annotation Location Component / Role(s)
His Tag
Start Codon
RBS
J23100
TAT
Linker
HA2
Linker
Apoptin
Stop Codon
51,68
48,50
36,47
1,35
69,101
102,146
147,215
216,260
261,623
624,629
feature/tag tag
start_codon feature/start
ribosome_entry_site feature/rbs
promoter feature/promoter
CDS feature/cds
feature/BioBrick engineered_region
CDS feature/cds
feature/BioBrick engineered_region
feature/protein CDS
stop_codon feature/stop
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BBa_K1202106/1