BBa_K1204019

BBa_K1204019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1204019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Baron
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:40

TEF1pr-BS3x-16A



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1204019_sequence (Version 1)

Description

Same as BBa_K1204013 except the binding site is repeated 3 times. This should create apparent cooperativity in the binding of the iTALs and gRNA/dCas9 complex

Notes

Had to consider binding rules for iTALs and gRNA/dCas9 complex. Also had to create spacers between repeated binding sites.

Source

Ordered as gene block from IDT

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1204019/1