BBa_K1206000

BBa_K1206000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1206000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anish Vaghela

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:09:40

LuxOperon -> qsdA with GFP as a reporter

Details

• Types: DnaRegion
• Roles: Project; engineered_region
• Sequences: BBa_K1206000_sequence (Version 1)

Description

The final project includes the LuxOperon and a bidirectional promoter that activates the qsdA degradation molecule for C6-HSL autoinducers.

Notes

We made the addition of a Glycine-Serine linker to add GFP as a reporter for the qsdA protein.

Source

The part was constructed using genscript gene synthesis services.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
luxI	1,579	CDS, feature/cds
BBa_C0061	1,618	engineered_region, feature/BioBrick
LVA	580,611	tag, feature/tag
Help:Barcodes	619,643	engineered_tag, feature/barcode
prefix	650,651	feature/BioBrick, engineered_region
luxR	650,1399	feature/cds, CDS
BBa_C0062	650,1405	engineered_region, feature/BioBrick
T	823,823	feature/s_mutation, point_mutation
A	1141,1141	point_mutation, feature/s_mutation
Help:Barcodes	1406,1430	engineered_tag, feature/barcode

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1206000/1

Member of these Collections

• iGEM Parts Registry