BBa_K1216007

BBa_K1216007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1216007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Meret Arter

Date created: 2013-09-20 11:00:00

Date modified: 2015-05-08 01:09:41

Variant of the wild-type pLuxR promoter with lower sensitivity

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1216007_sequence (Version 1)

Description

BBa_K1216007 is a variant of the wild-type pLuxR promoter with a lowered sensitivity for LuxR-AHL.

Notes

LuxR binding site were mutated to reduce the sensitivity of the promoter.

Source

The promoter was built through site-saturation mutagenesis of the wild-type promoter.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LuxR/HSL binding site	1,20	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1216007/1

Member of these Collections

• //promoter
• iGEM Parts Registry