Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K1218003_sequence (Version 1)
|
Description
The E. coli CRISPR system uses the CASCADE complex to target invasive DNA. CasA is the first subunit of the CASCADE complex and is responsible for the initial identification of invasive DNA. This part can be used with casBCDE to create the full CASCADE protein complex.
Notes
We had to perform mutagenesis twice to remove PstI cut sites.
Source
E. coli DH5alpha genome