BBa_K1218010

BBa_K1218010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1218010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Constantino
Date created: 2013-08-28 11:00:00
Date modified: 2015-05-08 01:09:42

Cas3 Endonuclease



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1218010_sequence (Version 1)

    Description

    An endonuclease that participates with the CASCADE protein complex during CRISPR-Cas interference in E. coli K-12.

    Notes

    Contained 2 internal PstI sites. We used site-directed mutagenesis to remove the sites without altering the amino acid sequence.

    Source

    Amplified from DH5α genomic DNA.

    Sequence Annotation Location Component / Role(s)
    start
    Cas3
    stop
    		1,3
    1,2667
    2665,2667
    		start_codon feature/start
    feature/protein CDS
    feature/stop stop_codon

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    rdf-schema#seeAlso
    P38036
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1218010/1
     

    Attachments

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