BBa_K1218019

BBa_K1218019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1218019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Constantino
Date created: 2013-08-29 11:00:00
Date modified: 2015-05-08 01:09:42

CASCADE Complex



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1218019_sequence (Version 1)

    Description

    This polycistronic sequence contains the CasABCDE genes that form the CASCADE complex. Functions in the innate CRISPR-Cas immune system of Escherichia coli.

    Notes

    Silent mutations were engineered via site-directed mutagenesis to remove two internal PstI sites.

    Source

    Amplified from DH5α genomic DNA.

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1218019/1
     

    Attachments

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