| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K123003_sequence (Version 1)
|
Description
This basic part is derived from the Human Estrogen Receptor Alpha and optimized for activity in E.Coli. When estrogen or an estrogen like compound is present the receptor binds to it and forms a homodimer. This homodimer is then able to bind to an Estrogen Responsive Element (ERE BBA_K123002). In Humans this estrogen receptor (ER) is a transcription factor that inciates transcription. However because we are interested in using this protien in bacteria we could not assume the same enhancer like effects. We have designed this part to act as a repressor when bound to the ERE by physically blocking the Polymerase
Notes
There is evidence that ER can be toxic to cells. However we found that while it does effect cell growth rate the toxicity was no lethal
Source
This Part originates as the Human Estrogen Receptor Alpha
